What is MS MS UPLC?
Ultra performance liquid chromatography – tandem mass spectrometer (UPLC-MS/MS) (UP)LC-MS/MS is a chemistry technique that combines the physical separation capabilities of liquid chromatography with the mass analysis capabilities of mass spectrometry.
What is the difference between MS and LC-MS?
LC-MS and LC-MS/MS are the combination of liquid chromatography (LC) with mass spectrometry (MS). MS means you only analyze your precursor ion (as generated in the source) for example in an iontrap, a quadrupole or time-of-flight Massspec. MS/MS is the combination of two mass analyzers in one mass spec instrument.
What is LC-MS machine?
Liquid chromatograph mass spectrometer (LC/MS) is, as its name suggests, a device that is a combination of a liquid chromatograph (LC) and a mass spectrometer (MS). The LC/MS is used in a wide range of areas. A gas chromatograph mass spectrometer (GC/MS) is an analytical instrument for volatile substances.
How does LC-MS machine work?
The mass spectrometer for an LC/MS/MS system has an ionization source where the LC column effluent is nebulized, desolvated and ionized creating charged particles. These charged particles then migrate under high vacuum through a series of mass analyzers (quadrupole) by applying electromagnetic fields.
What industries use LC-MS?
LCMS is applied in many industries such as pharmaceuticals, biopharmaceuticals, forensic, industrial, food and environmental sector. For clinical research, the analysis of drugs, vitamins and minerals in whole blood, plasma, serum and urine is conducted routinely using LCMS.
What is UPLC vs HPLC?
A: Both HPLC and UPLC are both liquid chromatography techniques used to separate the different components found in mixtures. However, UPLC operates at higher pressures (15,000 psi) and allows for lower particle sizes in columns, while HPLC operates at lower pressures (max <6000 psi).