What is the difference between downstream and upstream processing?
It’s not unusual to hear people involved in production make frequent reference to “upstream” and “downstream” parts of the process. Upstream refers to the material inputs needed for production, while downstream is the opposite end, where products get produced and distributed.
What is downstream process in fermentation?
Downstream processing involves all unit operations after fermentation that improve the purity of the final ethanol product. Ethanol is typically purified using combinations of distillation and molecular sieving. The fermented broth from the beer well is sent to a traditional distillation column to obtain 95% ethanol.
What is upstream and downstream process development?
Upstream processes are those in which biological materials are either obtained from an outside source or inoculated and grown in culture, under controlled conditions, to manufacture certain types of products. Downstream processes are those in which the products are harvested, tested, purified and packaged.
What is the aim of upstream processing?
The goal of upstream biomanufacturing is to grow cells that will produce a desired protein, the protein being further purified during the downstream processing steps (see Downstream Processing chapter).
What is upstream and downstream fermentation?
The key difference between upstream and downstream bioprocessing is that upstream bioprocessing involves screening and identification of microorganisms, media preparation, multiplication of microbes inside a bioreactor, and incubation, while downstream bioprocessing involves extraction, purification and packaging of …
What is upstream processing and downstream processing?
Upstream & Downstream Processing. The production of active pharmaceutical ingredients (API) of biopharmaceuticals typically starts with generation of the API via living cells known as the upstream process, followed by purification of the target API, or the downstream process.
What is an upstream process?
Upstream process is the first step of bioprocess from early cell isolation and cultivation, to cell banking and culture development of the cells until final harvest where the desired quantity is reached. Since this is the early stage of bioprocessing, the quality of the product is of critical importance.
What is the Upstream phase of the fermentation process?
Included in the upstream phase is the fermentation process itself which usually is carried out in large tanks known as fermenters or bioreactors.
What are the stages of industrial fermentation operation?
Any industrial fermentation operation can be broken down into three main stages, viz, upstream processing, the fermentation process and downstream processing. Upstream processing includes formulation of the fermentation medium, sterilisation of air, fermentation medium and the fermenter, inoculum preparation and inoculation of the medium.
What are the steps involved in upstream processing?
Upstream processing includes formulation of the fermentation medium, sterilisation of air, fermentation medium and the fermenter, inoculum preparation and inoculation of the medium. The fermentation medium should contain an energy source, a carbon source, a nitrogen source and micronutrients required for the growth of the microorganism along
What are the fermentation factors that affect downstream processing?
Fermentation factors affecting downstream processing include the properties of microorganisms, particularly morphology, flocculation characteristics, size and cell wall rigidity. These factors have major influences on the filterability, sedimentation and homogenization efficiency.