What is CMV enhancer?
The cytomegalovirus (CMV) major immediate early (MIE) enhancer-containing promoter regulates the expression of the downstream MIE genes, which have critical roles in reactivation from latency and acute infection. The enhancer consists of binding sites for cellular transcription factors that are repeated multiple times.
Do you need CMV enhancer?
The CMV promoter does not require the enhancer region, but the enhancer is frequently included in many plasmids. As an alternative, you might consider expressing NRF2 and GFP as a fusion protein, circumventing the need for anothe promoter.
What is AmpR promoter?
AmpR promoter is the promoter for ampicillin resistance. It is a weak promoter. Any gene can also be cloned under this promoter and expressed in bacteria like E. Coli. There is a pCutamp plasmid used for plasmid-curing in Escherichia coli by targeting the AmpR promoter.
What is SFFV promoter?
SFFV promoter provides the best level of transgene expression in Raji cells and LCLs. Since CMV and EF1α promoters allowed for ≤40.25% transgene expression in Raji cells further experiments were conducted using the SFFV promoter.
What is a PGK promoter?
The Pgk-1 promoter is in a region rich in nucleotides G and C. This promoter can efficiently drive high levels of expression of reporter genes such as E. coli lacZ and neo. We have determined that the 120 bp upstream of the transcription start site functions as a core promoter.
What are minimal promoters?
Dear researcher, minimal promoter refers to a minimal sequence of a native promoter (mostly core promoter) that could express a downstream gene. So, that not mean minimal promoters’ expression is low or in minimal level. CMV and SV40 are virus derived promoters and they are so strong.
What is AMPR and why is it important?
ampR is a master regulatory gene that switches the expression of hundreds of other genes on and off, including genes involved in antibiotic resistance. This gene acts as an evolutionary catalyst for antibiotic resistance.
What protein does AMPR code for?
The ampr gene of Escherichia coli and other Gram-negative bacteria codes for a /3-lactamase (penicillin amido-3-lac- tamhydrolase, EC 3.5. 2.6) of approximately 27,000 daltons that catalyzes the hydrolysis of penicillins to penicilloic acids.
How do you silence CMV promoter?
The cytomegalovirus (CMV) promoter, a strong promoter often used for transgene expression in mammalian cells in culture, is known to be silenced by DNA methylation and histone deacetylation but there is no report on the role of histone methylations in its regulation.
What is SFFV?
The spleen focus-forming virus (SFFV) envelope gene, when introduced into mice in the absence of other SFFV genes, induces acute erythroleukemia.
What is spleen focus-forming virus?
The Friend spleen focus-forming virus (SFFV) is a highly pathogenic retrovirus that exclusively induces erythroleukemia in susceptible strains of mice within weeks of inoculation (for review, see ref. 1).
What is the difference between an operator and an enhancer?
In the Operon Model, the operator is the gene segment to which a repressor binds. This prevents the RNA polymerase from transcribing certain genes in the operon unit. Enhancer DNA sequences bind transcription factors called enhancer-binding proteins which increase the rate of transcription.