What is acid phosphatase enzyme activity?
Acid phosphatase activity is more than 200 times more abundant in prostatic tissue than in any other tissue and is the source of the high levels of acid phosphatase in ejaculate. Phosphatase enzymes hydrolyze many types of organic monophosphate esters to yield inorganic phosphate and alcohol.
What is seminal acid phosphatase?
Acid phosphatase (AP) is an enzyme secreted by the prostate gland into seminal fluid and is found in concentrations up to 400 times greater in semen than in other body fluids (Sensabaugh 1979, Saferstein 2001).
Why is acid phosphatase test important?
The acid phosphatase test forensics helps in the identification of seminal stains in rape cases. Semen is known to contain the acid phosphatase enzyme in abundance.
What causes high acid phosphatase?
Prostatic acid phosphatase (PAP), which is produced in the prostate, was the first major serum marker for prostate cancer. Metastasis of prostate cancer beyond the capsule, particularly to the bone, causes a rise in acid phosphatase level, with the level increasing in correspondence to the extent of the disease.
What is the difference between alkaline and acid phosphatase?
The key difference between acid phosphatase and alkaline phosphatase is that acid phosphatase is a phosphatase enzyme that is optimally active in an acidic pH, while alkaline phosphatase is a phosphatase enzyme that is optimally active in an alkaline pH.
What is acid and alkaline phosphatase?
Acid phosphatase is a phosphatase enzyme that is optimally active in an acidic pH, while alkaline phosphatase is a phosphatase enzyme that is optimally active in an alkaline pH. So, this is the key difference between acid phosphatase and alkaline phosphatase.
Why is alkaline phosphatase called alkaline?
Alkaline phosphatase (ALP, ALKP, ALPase, Alk Phos) (EC 3.1. 3.1), or basic phosphatase, is a homodimeric protein enzyme of 86 kilodaltons. Each monomer contains five cysteine residues, two zinc atoms and one magnesium atom crucial to its catalytic function, and it is optimally active at alkaline pH environments.
How is phosphatase enzyme prepared for the assay?
Dilute samples of protein phosphatase to 50 µl in 1x colorimetric assay buffer in 96-well plate at room temperature. Prepare 10 mM pNPP substrate solution in 1x colorimetric assay buffer. Add 50 µl pNPP solution to each well at regular time intervals (10s). Let reaction proceed for 10–45 min at room temperature (RT).
Where is acid phosphatase found in the cell?
According to the former, the enzyme appears to be almost entirely (95 per cent) located in the cytoplasm, while according to the latter, the acid phosphatase is predominantly concentrated in the nuclei and in some peribiliary polymorphic structures identified as myelin figures.
Is phosphatase an enzyme?
Phosphatases are the extracellular enzymes that catalyze the hydrolysis of phospho-ester bonds in organic P-containing substrates releasing inorganic P in the form of orthophosphates that can be used by soil biota and plants.