What is Engelbreth-Holm-Swarm mouse sarcoma?
The Engelbreth-Holm-Swarm (EHS) sarcoma is a rich source of both individual basement membrane components and Matrigel. Maintenance of the EHS tumor in mice and its subsequent harvesting for the preparation of Matrigel are described.
Is Matrigel a synthetic extracellular matrix?
Matrigel is a commonly used cell culture matrix, consisting of natural polymers secreted by Engelbreth-Holm-Swarm (EHS) mouse sarcoma cells. It contains critical growth factors and cytokines for cell growth [282,283].
What is Engelbreth-Holm-Swarm?
Engelbreth-Holm-Swarm (EHS) tumor produces large amounts of basement membrane (BM) components, which are widely used as cell culture substrates mimicking BM functions. EHS tumor arose spontaneously in an ST/Eh strain mouse and has been propagated by transplantation.
What is Matrigel matrix?
Corning Matrigel matrix is a reconstituted basement membrane extracted from EHS mouse tumor. When the material is extracted from the tumor, it contains laminin, collagen IV, entactin, heparan sulfate proteoglycan, and growth factors that occur naturally in the EHS tumor.
Why is Matrigel used?
A gelatinous protein mixture derived from mouse tumor cells and commercialized as Matrigel is commonly used as a basement membrane matrix for stem cells because it retains the stem cells in an undifferentiated state.
What is the difference between Matrigel and Geltrex?
hESC-qualified = doesn’t cause human embryonic stem cell differentiation….Similarities.
| Feature | Matrigel + Geltrex |
|---|---|
| Cell types they work for: | List for each is extensive so check your individual cell type works with each manufacturer and ask them to recommend a formula. |
| hESC-qualified: | For some formulations. |
How long do Matrigel coated plates last?
one week
Storage of Matrigel® Coated Plates Coated plates can be stored at 2-8 °C for up to one week (sealed with Parafilm™ to prevent dehydration) before use.
What is Ldev-free Matrigel?
Corning™ Matrigel™ Basement Membrane Matrix, LDEV-Free is tumor rich in extracellular matrix proteins, including Laminin (a major component), Collagen IV, heparin sulfate proteoglycans, entactin/nidogen, and a number of growth factors.