What are the recognition sequence for PvuII?
The DNA methyltransferase (M·PvuII) catalyzes the transfer of a methyl group from the donor S-adenosyl-l-methionine to the N4 position of the internal cytosine in the recognition sequence (CAGN4mCTG) (15,18).
What is the recognition site for pBR322?
So, the correct answer is ‘BamHl’.
What are restriction sites in pBR322?
pBR322 contains multiple restriction sites including sites for BamHI, HindIII, SalI, PvuI, PvuII, PstI, EcoRI and ClaI restriction enzymes.
What is the purpose of restriction mapping?
Restriction mapping is a method used to map an unknown segment of DNA by breaking it into pieces and then identifying the locations of the breakpoints. This method relies upon the use of proteins called restriction enzymes, which can cut, or digest, DNA molecules at short, specific sequences called restriction sites.
Which selectable marker is incorporated in pBR322 plasmid?
The pBR322 plasmid is a commonly used cloning vector that contains both the ampicillin and tetracycline resistance genes as selectable markers. Escherichia coli cells transformed with pBR322 plasmids have a selective advantage in antibiotic-treated environments.
How to map the relative positions of restriction sites in plasmids?
In this experiment, a plasmid DNA is cleaved with different combinations of restriction enzymes. By determining the fragment size and using agarose gel electrophoresis, the relative positions of the restriction sites can be mapped. • A restriction enzyme lab with a strong analysis component!
What are the applications of restriction enzymes in biology?
Although restriction enzymes are widely used in molecular cloning, their use as molecular tools extends to other common applications in molecular biology. Two important applications are DNA fingerprinting and methylation analysis, which are methods to map sequences and analyze epigenetic patterns in the genome.
What is the role of restriction enzymes in the study of CpG islands?
CpG islands are intensively studied because of the potential role of their methylation state (s) in the inactivation and activation of gene transcription. Among methods available to investigate DNA methylation state, restriction enzymes are a popular tool due to their availability and ease of use.
What is the purpose of restriction endonuclease mapping?
The use of restriction endonuclease mapping (gene mapping) is a tool for analysing genetic disease. DNA obtained from tissues or cells (usually peripheral blood leucocytes) is treated with restriction enzymes and analysed by Southern blotting using a radioactively labelled gene probe.