What is goat anti-mouse IgG?
Unconjugated Goat Anti-Mouse IgG Antibody binds to mouse primary antibodies for immunohistochemistry and many other applications. Features: Affinity-purified, ultrapure, high affinity antibody. Thoroughly adsorbed against serum and immunoglobulins from potentially interfering species.
What does goat anti-mouse antibody mean?
Anti-mouse secondary antibodies are generated by immunizing a host animal (e.g., a goat) with a pooled population of immunoglobulins (Ig) from the target species (i.e., mice). After the host animal’s immune system responds to produce anti-mouse Ig antibodies, serum is collected, and the target antibodies are purified.
What is goat anti rabbit IgG?
Goat anti rabbit IgG antibody recognizes the Fc region of rabbit immunoglobulin G, confirmed by ELISA and immunoelectrophoresis. The antibody has not been species cross adsorbed and may cross react with IgG from other species. It shows weak recognition of rabbit IgM in ELISA.
What is HL antibody?
Heavy. Chain. Anti-IgG (H+L): A secondary antibody with the (H+L) designation targets the heavy and light chains of the IgG molecule (i.e. Fc and Fab regions). These antibodies also react with other classes (e.g. IgE, IgD, etc.) due to shared light chains between classes.
What does IgG h l mean?
What is the role of goat anti-rabbit IgG horseradish peroxidase conjugate in the experiment?
Goat anti-Rabbit IgG Secondary Antibody [HRP (Horseradish Peroxidase)] Summary. Detects rabbit IgG heavy and light chains in direct ELISAs and Western blots. In direct ELISAs, less than 5% cross-reactivity with human IgG, mouse IgG, and chicken IgY is observed.
What is normal goat serum?
Normal Sera are non-immune serum samples from goat, human, mouse, rabbit and other species that contain the usual complement of serum proteins, including the various classes of immunoglobulins that exist in healthy animals of the particular species.
What is the difference between IgG2A and IgG2B?
IgG2A is a representative of the canonical Y-shaped IgG molecule with the disulfide bonds of the Fab portion being independent of those in the hinge. IgG2B is more constrained due to the Fab arms being covalently attached to the hinge via disulfide bonds and can be depicted as a T-shaped molecule.
What is the human anti-mouse antibody test for?
The HAMA response. Antibody treatment is a type of therapy that is used to treat certain types of cancer and immune disorders. Antibodies are proteins which are naturally formed by the body in response to a foreign substance, known as an antigen.
What is the secondary antibody for mouse monoclonal?
Most primary antibodies are produced in mouse or rabbit hosts; therefore, anti-mouse IgG and anti-rabbit IgG are the most popular types of secondary antibodies. Goat is the host species most easily and frequently used by manufacturers to produce polyclonal anti-mouse and anti-rabbit secondary antibodies.
What does H l mean in antibodies?
(H+L) Heavy + light. Whole immunoglobulin (heavy and light chain)
Which of the following molecule’s can be detected by ELISA?
ELISA tests primarily detect proteins (as opposed to small molecules and ions such as glucose and potassium).
Why is goat serum used?
Normal goat serum ab7481 is used extensively for the blocking of non-specific antibody binding in tissue and cell staining, and in other applications of antibodies.
Can goat antibodies cross adsorb mouse immunoglobulin?
Goat anti Mouse IgG (H/L) polyclonal antibody reacts with the heavy chains of mouse IgG, and the light chains common to the majority of mouse immunoglobulins. This antibody has not been cross adsorbed against other mouse immunoglobulins, and has been found to react substantially with antibody light chains common to all mouse antibodies.
How much antibody is needed to detect Precision Plus protein prestige standards?
Precision Plus Protein™ Prestained Standards were run in lane 1. 1 μg and 0.25 μg of antibody was run in lanes 2, 4 and 6 and lanes 3, 5 and 7 respectively. Protein detection was carried out on lanes 2 and 3 using Goat anti Mouse IgG (H/L):HRP ( STAR207P) at a dilution of 1/10,000.
Does this antibody cross-react with other immunoglobulins?
This antibody has been solid-phase absorbed to ensure minimal cross-reaction with rat, human, bovine, horse, and rabbit immunoglobulins, but it may have minimal cross-reactivity with other subclasses of mouse immunoglobulins. Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Can I view information about IgG on Uniprot?
View information about IgG on UniProt.org For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.